Journal: Frontiers in Immunology

Article Title: PhIP-driven prostate cancer involves key molecular regulators and immune microenvironment modulation

doi: 10.3389/fimmu.2026.1782240

Figure Lengend Snippet: PhIP exposure induces cytotoxicity in RWPE-1 cells and downregulates SLC14A1 expression in PC-3 cells. (A) Immunohistochemical staining of SLC14A1 in prostate cancer tissues and normal prostate tissues based on data from the HPA database. (B) Cell viability of RWPE-1 cells after treatment with increasing concentrations of PhIP for 48 h, as determined by CCK-8 assay. (C) Protein expression levels of SLC14A1 in PC-3 cells following PhIP treatment, as assessed by Western blot. (D) Relative mRNA expression levels of SLC14A1 in PC-3 cells after PhIP treatment, as determined by qRT-PCR (**p < 0.01, ***p < 0.001).

Article Snippet: The human prostatic epithelial cell line RWPE-1 and the human prostate cancer cell line PC-3 were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing, Immunohistochemical staining, Staining, CCK-8 Assay, Western Blot, Quantitative RT-PCR

Journal: bioRxiv

Article Title: IL12-engineered human PSMA-CAR T cells for the treatment of advanced prostate cancer

doi: 10.64898/2026.03.05.709907

Figure Lengend Snippet: Engineering hPSMA-CARs with mbIL12 rescues CAR T cell functionality a Schema of hPSMA-CAR variants with mbIL12 expression. b Flow cytometric analysis of ET260-1 CAR and ET260-1(58) CAR with or without mbIL12 as detected by extracellular Fc and intracellular IL-12. c Tumor cell killing of hPSMA-CARs at an E:T of 1:4 over 72 hrs. d Expression of 4-1BB over 72 hrs on hPSMA-CARs. e Expression of CD25 on hPSMA-CARs after 72 hrs. f Secretion of IFNγ of hPSMA-CARs with mbIL12 after 72 hrs as determined by ELISA. g Tumor cell killing of hPSMA-CARs with mbIL12 and J591 against PC-3 PIP at E:T of 1:10 over 5 days at varying concentrations of anti-IFNγR1 blocking antibody and isotype control. Data are presented as mean values ± SEM. P values indicate differences between ET260-1-dCH2(28tm)BBz and ET260-1-dCH2(28tm)BBz/mbIL12 and between ET260-1-dCH2(28tm)BBz/mbIL12 and J591-dCH2(4tm)BBz using a two-tailed Student’s t-test.

Article Snippet: Human metastatic prostate cancer cell line PC-3 (ATCC CRL-1435) was cultured in RPMI-1640 (Corning) containing 10% fetal bovine serum (FBS, Hyclone), and 1X antibiotic-antimycotic (AA, Gibco) (complete RPMI).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Blocking Assay, Control, Two Tailed Test

Journal: bioRxiv

Article Title: IL12-engineered human PSMA-CAR T cells for the treatment of advanced prostate cancer

doi: 10.64898/2026.03.05.709907

Figure Lengend Snippet: mbIL12-engineered hPSMA-CAR T cells demonstrate improved activity in a recursive tumor cell assay in vitro a Tumor cell killing of hPSMA-CARs over 8 days when co-cultured with PSMA-expressing cell lines at E:T of 1:20. b Fold expansion of hPSMA-CARs compared to J591-CAR after 8-day co-culture. c Production of IFNγ at 8 days as determined by ELISA. d Schema of rechallenge with PSMA-CARs co-cultured with PC-3 PSMA lo , PC-3 PSMA hi , or PC-3 PIP and rechallenged with tumor cells every 3 days. e PC-3 PSMA lo (left), PC-3 PSMA hi (center), or PC-3 PIP (right) cell counts were quantified by flow cytometry. b Fold expansion of T cells after each rechallenge were quantified by flow cytometry. c Production of IFNγ by T cells following each rechallenge as determined by ELISA. Data are presented as mean values ± SEM. P values indicate differences between ET260-1-dCH2(28tm)BBz and ET260-1-dCH2(28tm)BBz/mbIL12 and between ET260-1-dCH2(28tm)BBz/mbIL12 and J591-dCH2(4tm)BBz using a two-tailed Student’s t-test.

Article Snippet: Human metastatic prostate cancer cell line PC-3 (ATCC CRL-1435) was cultured in RPMI-1640 (Corning) containing 10% fetal bovine serum (FBS, Hyclone), and 1X antibiotic-antimycotic (AA, Gibco) (complete RPMI).

Techniques: Activity Assay, In Vitro, Cell Culture, Expressing, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Two Tailed Test